The BeeConnected SUDOE project addresses some common challenges in the SUDOE area related to habitat fragmentation and lack of connectivity, primarily due to agricultural intensification and urban expansion, factors that threaten European biodiversity and, especially, wild pollinators. In line with the recent “European Union Pollinators Initiative: A New Deal for Pollinators,” BeeConnected SUDOE focuses on the creation and restoration of green infrastructures for pollinators (“Buzz Lines”), which mitigate territorial fragmentation and strengthen connectivity between the populations of these organisms. BeeConnected SUDOE designs actions and tests ecological restoration strategies in four pilot areas, to address the lack of connectivity of wild pollinators in fragmented agricultural and peri-urban landscapes, in line with the principles of the European Green Deal. The project activities focus on: i) restoring degraded areas to improve their role in territorial connectivity; ii) increasing food, water, and nesting resources for pollinators; iii) designing awareness and communication campaigns on the importance of pollinator conservation and green infrastructures. The main innovation of the project lies in its comprehensive approach, which combines scientific research, community participation, and the implementation of nature-based solutions. Transnational cooperation is key to the project’s success, as it addresses challenges that go beyond national borders, such as the decline of pollinators and the promotion of nature-based solutions. BeeConnected SUDOE facilitates collaboration between academic entities and public authorities, implementing strategies that contribute to territorial rebalancing, improve ecological connectivity between urban and rural areas, and foster public awareness about the importance of biodiversity conservation.